Difference between revisions of "Part:BBa K1061013"

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We also get the quantitive data by western blot, which will be submitted later.
 
We also get the quantitive data by western blot, which will be submitted later.
 
====quantitive analysis====
 
====quantitive analysis====
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<h2>Measurement Contribution</h2>
  
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< Uncomment this to enable Functional Parameter display  
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*'''Group:''' Tsinghua-A, 2016
 +
*'''Author:''' Yuxi Ke
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*'''Summary:''' We quantitatively measured and calculated TRE promotor's ability to accurately transmit information by channel capacity, a concept in information theory, the unit of which is bit. Channel capacity evaluates correlation between input and output signals. For instance, if channel capacity is larger than 1 bit, the part can decern between high and low inputs, and vice versa.
 +
*the documentation of your improvement (text, graphs, pictures, tables, etc.)
 +
**Include sections for purpose, results, methods, etc. (if applicable)
 +
*'''Uploads:''' (links to uploads relevant to your contribution, ex: csv containing your data, sequence files, etc.) '''Note:''' All files pertaining to a contribution must be [[Help:Upload_Files|uploaded to the Registry]].
 +
 
 +
< !-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K1061013 parameters</partinfo>
 
<partinfo>BBa_K1061013 parameters</partinfo>
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<p>
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<h2>References:</h2>
 
<h2>References:</h2>
 
<p>[1]http://www.clontech.com/CN/Products/Inducible_Systems/Tetracycline-Inducible_Expression/Gen2?sitex=10022:22372:US
 
<p>[1]http://www.clontech.com/CN/Products/Inducible_Systems/Tetracycline-Inducible_Expression/Gen2?sitex=10022:22372:US

Revision as of 02:18, 15 October 2016

P tight promoter

It is a response element containing 7 consecutive TetO elements and a minimal CMV promoter. When binding to tTA or rtTA, the expression of the downstream gene will be highly activated. Besides, it can achieve low constitutive expression(almost undectectable) when not binding to tTA or rtTA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Funtional experiment

We conducted the functional experiment by co-tranfected the plasmid contains tTA and the plasmid contains P tight into Bosc, one kind of HEK-293 cell lines.Noted that we did not use tTA advance due to time limit(we construct the plasmid contains tTA advance later), and we will try that later,for improvement of expression control.

functional assay of tTA

The after adding dox, the expression of dox is almost fully suppressed. We also get the quantitive data by western blot, which will be submitted later.

quantitive analysis

Measurement Contribution

  • Group: Tsinghua-A, 2016
  • Author: Yuxi Ke
  • Summary: We quantitatively measured and calculated TRE promotor's ability to accurately transmit information by channel capacity, a concept in information theory, the unit of which is bit. Channel capacity evaluates correlation between input and output signals. For instance, if channel capacity is larger than 1 bit, the part can decern between high and low inputs, and vice versa.
  • the documentation of your improvement (text, graphs, pictures, tables, etc.)
    • Include sections for purpose, results, methods, etc. (if applicable)
  • Uploads: (links to uploads relevant to your contribution, ex: csv containing your data, sequence files, etc.) Note: All files pertaining to a contribution must be uploaded to the Registry.

< !-- Uncomment this to enable Functional Parameter display

Functional Parameters

n/aP tight promoter

References:

[1]http://www.clontech.com/CN/Products/Inducible_Systems/Tetracycline-Inducible_Expression/Gen2?sitex=10022:22372:US