Difference between revisions of "Part:BBa K2070016"

Revision as of 07:04, 25 October 2016

Constitutive ecf16 generator

This part contains an ECF16 coding region downstream of a medium rbs(BBa_B0032) and strong promoter (BBa_J23101). The ecf16 generated can activate the ecf16 promoter (BBa_K2070000).

Usage and Biology

Photograph of plates containing cells transformed with sigma16 related constructs under blue light. From bottom left: BBa_K2070006 (ECF sigma16 inducible GFP reporter), BBa_K2070026 (Constitutive ecf16 generator with ecf 16 inducible GFP reporter), BBa_K2070016 (Constitutive ECF sigma16 generator). As only cells with BBa_K2070026 is shown to fluorescence, it can be suggested that the devices works as expected.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 239
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 434
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
 The ecf16 generated can activate the ecf16 promoter (<partinfo>BBa_K2070000</partinfo>).
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+[[File:T--UT-Tokyo--BBa K2070000--sigma1601.png|thumb|500px|'''Photograph of plates containing cells transformed with sigma16 related constructs under blue light.'''
+From bottom left: BBa_K2070006 (ECF sigma16 inducible GFP reporter), BBa_K2070026 (Constitutive ecf16 generator with ecf 16 inducible GFP reporter), BBa_K2070016 (Constitutive ECF sigma16 generator). As only cells with BBa_K2070026 is shown to fluorescence, it can be suggested that the devices works as expected.]]
-<!-- -->
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K2070016 SequenceAndFeatures</partinfo>