Difference between revisions of "Part:BBa K2100023"
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<partinfo>BBa_K2100023 short</partinfo> | <partinfo>BBa_K2100023 short</partinfo> | ||
− | The entry vector contains a promoter repressible by the | + | The entry vector contains a promoter repressible by the TAL21 repressor. pTAL21 consists of one repressible site upstream and four repressible sites downstream of the minimal CMV promoter. (Li et al., 2015) [1] A VP-16 Gal4 protein complex recruits the transcription machinery of the promoter. The TAL21 repressor inhibits the activator and transcription machinery from transcribing the gene downstream of pTAL21. |
+ | [1] Li, Y., Jiang, Y., Chen, H., Liao, W., Li, Z., Weiss, R., & Xie, Z. (2015). Modular construction of mammalian gene circuits using TALE transcriptional repressors. Nature Chemical Biology, 11(3), 207–213. http://doi.org/10.1038/nchembio.1736 | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== |
Latest revision as of 01:26, 15 October 2016
pENTR pTALER21
The entry vector contains a promoter repressible by the TAL21 repressor. pTAL21 consists of one repressible site upstream and four repressible sites downstream of the minimal CMV promoter. (Li et al., 2015) [1] A VP-16 Gal4 protein complex recruits the transcription machinery of the promoter. The TAL21 repressor inhibits the activator and transcription machinery from transcribing the gene downstream of pTAL21.
[1] Li, Y., Jiang, Y., Chen, H., Liao, W., Li, Z., Weiss, R., & Xie, Z. (2015). Modular construction of mammalian gene circuits using TALE transcriptional repressors. Nature Chemical Biology, 11(3), 207–213. http://doi.org/10.1038/nchembio.1736 Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 182
Illegal XbaI site found at 157 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 182 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 182
Illegal BamHI site found at 311
Illegal XhoI site found at 248 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 182
Illegal XbaI site found at 157 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 182
Illegal XbaI site found at 157 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 326