Difference between revisions of "Part:BBa K2109110"
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<partinfo>BBa_K2109110 short</partinfo> | <partinfo>BBa_K2109110 short</partinfo> | ||
− | + | HDVR U1A-C75T is a functional ribozyme in the presence of 1% xylose positive control. It can be in vitro transcribed via T7-RNA Polymerase to produce a transcript of approximately ~250 bp. The other HDVR negative control U1A (Part:BBa_K2109109)is inducible in the presence of 1% xylose and also produces of transcript of ~250 bp when in vitro transcribed. | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Revision as of 18:33, 27 October 2016
HDVR U1A-C75T
HDVR U1A-C75T is a functional ribozyme in the presence of 1% xylose positive control. It can be in vitro transcribed via T7-RNA Polymerase to produce a transcript of approximately ~250 bp. The other HDVR negative control U1A (Part:BBa_K2109109)is inducible in the presence of 1% xylose and also produces of transcript of ~250 bp when in vitro transcribed.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 58
Illegal NgoMIV site found at 87 - 1000COMPATIBLE WITH RFC[1000]