Difference between revisions of "Part:BBa K1981101"
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AI-2 is generated by many species of Gram-negative and Gram-positive bacteria. In a group of bacteria exemplified by E.coli MG1655, AI-2 response involves lsr gene clusters that encode lsrACDB, lsrK, lsrFG. Plsr is the promoter of the lsr operon. | AI-2 is generated by many species of Gram-negative and Gram-positive bacteria. In a group of bacteria exemplified by E.coli MG1655, AI-2 response involves lsr gene clusters that encode lsrACDB, lsrK, lsrFG. Plsr is the promoter of the lsr operon. | ||
| − | [[Image:NKU China AI-2-luxS signal pathway in E-coli.png| | + | [[Image:NKU China AI-2-luxS signal pathway in E-coli.png|600px|thumb|center|'''Figure 1:''' Schematic overview of the AI-2 response pathway in E.coli MG1655]] |
===Characterization=== | ===Characterization=== | ||
AI-2 Response Device consists of the AI-2 quorum sensor-inducible promoter BBa_K1981101(249), a GFP coding sequence BBa_E0040(747bp), a double terminator BBa_B0015(115). The total length of AI-2 Response Device A is 1111bp. | AI-2 Response Device consists of the AI-2 quorum sensor-inducible promoter BBa_K1981101(249), a GFP coding sequence BBa_E0040(747bp), a double terminator BBa_B0015(115). The total length of AI-2 Response Device A is 1111bp. | ||
| − | [[Image:NKU China lsr Promoter 3 hour curve.png|800px|thumb|center|'''Figure | + | [[Image:NKU China lsr Promoter 3 hour curve.png|800px|thumb|center|'''Figure 2:''' Colony PCR Verification for AI-2 Response Device A.]] |
AI-2 Response Device consists of the AI-2 quorum sensor-inducible promoter BBa_K1981101(249), a GFP coding sequence BBa_E0040(747bp), a double terminator BBa_B0015(115). The total length of AI-2 Response Device A is 1111bp. | AI-2 Response Device consists of the AI-2 quorum sensor-inducible promoter BBa_K1981101(249), a GFP coding sequence BBa_E0040(747bp), a double terminator BBa_B0015(115). The total length of AI-2 Response Device A is 1111bp. | ||
[[Image:NKU China exogenously Added AI-2 On DeviceA NKU China.png|800px|thumb|center|'''Figure 1:''' GFP expression of AI-2 Response Device A when add exogenous AI-2.]] | [[Image:NKU China exogenously Added AI-2 On DeviceA NKU China.png|800px|thumb|center|'''Figure 1:''' GFP expression of AI-2 Response Device A when add exogenous AI-2.]] | ||
Revision as of 00:02, 15 October 2016
lsr promoter of LuxS/AI-2 signaling pathway in E.coli
Quorum sensing is a process of bacterial cell-to-cell communication involving the production and detection of extracellular signaling molecules called autoinducers. And autoinducer-2 (AI-2) has been proposed to serve as a 'universal signal' for interspecies communication. In the LuxS/AI-2 signaling system of E.coli MG1655, AI-2 response involves ATP binding cassette transporter encoded by genes named Lsr (LuxS regulated). Plsr is the promoter of the lsr operon. In our project, we use it to trigger the expression of a reporter gene， GFP.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
AI-2 is generated by many species of Gram-negative and Gram-positive bacteria. In a group of bacteria exemplified by E.coli MG1655, AI-2 response involves lsr gene clusters that encode lsrACDB, lsrK, lsrFG. Plsr is the promoter of the lsr operon.
Characterization
AI-2 Response Device consists of the AI-2 quorum sensor-inducible promoter BBa_K1981101(249), a GFP coding sequence BBa_E0040(747bp), a double terminator BBa_B0015(115). The total length of AI-2 Response Device A is 1111bp.
AI-2 Response Device consists of the AI-2 quorum sensor-inducible promoter BBa_K1981101(249), a GFP coding sequence BBa_E0040(747bp), a double terminator BBa_B0015(115). The total length of AI-2 Response Device A is 1111bp.
