Difference between revisions of "Part:BBa K2100023"
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<partinfo>BBa_K2100023 short</partinfo> | <partinfo>BBa_K2100023 short</partinfo> | ||
− | + | The entry vector contains a promoter repressible by the TAL14 repressor. pTAL14 consists of one repressible site upstream and four repressible sites downstream of the minimal CMV promoter. A VP-16 Gal4 protein complex is recruited to aid the transcription machinery of the promoter. The TAL14 repressor inhibits the activator and transcription machinery from transcribing the gene downstream of pTAL14. | |
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Revision as of 01:13, 15 October 2016
pENTR pTALER21
The entry vector contains a promoter repressible by the TAL14 repressor. pTAL14 consists of one repressible site upstream and four repressible sites downstream of the minimal CMV promoter. A VP-16 Gal4 protein complex is recruited to aid the transcription machinery of the promoter. The TAL14 repressor inhibits the activator and transcription machinery from transcribing the gene downstream of pTAL14.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 182
Illegal XbaI site found at 157 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 182 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 182
Illegal BamHI site found at 311
Illegal XhoI site found at 248 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 182
Illegal XbaI site found at 157 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 182
Illegal XbaI site found at 157 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 326