Difference between revisions of "Part:BBa K777000:Experience"

Revision as of 22:53, 14 October 2016

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UNIQeff0444142aee466-partinfo-00000000-QINU

No review score entered. Technion team 2016

GFP labeling is a very common way to examine the migration and location of certain proteins inside a living cell. here you can see such labeling in order to examine the migration of Tar chemoreceptor to the poles of E.coli bacteria.This property is critical for signal amplification and adaptation of the cell.

design this part is a composite of 3 parts from the iGEM registry. the Tar receptor (K777000) and GFP(B0040) were taken were amplified from the kit and the flexible linker (J18921) was add by using reverse transcription PCR.

experiments and results the fused Tar-GFP protein was cloned to our expression systems (K1992008,K1992009) in order to examine the migration of the receptor. By using a fluorescence microscope it can be seen in figure 1 that the receptor is closter in the poles of the bacteria as expected.

Figure 1

Fig1. (a) Tar-GFP expression in UU1250 strain cloned with K1992008 expression system. (b) Tar expression in UU1250 strain cloned with K1992004 expression system.

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 Fig1. (a) Tar-GFP expression in UU1250 strain cloned with K1992008 expression system. (b) Tar expression in UU1250 strain cloned with K1992004 expression system.
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