Difference between revisions of "Part:BBa K1992008"

 
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<partinfo>BBa_K1992008 short</partinfo>
 
<partinfo>BBa_K1992008 short</partinfo>
  
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This device expresses Tar chemoreceptor fused with GFP.
 
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===Usage and Biology===
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1992008 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1992008 SequenceAndFeatures</partinfo>
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==Introduction==
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A GFP gene was fused to the improved expression system of the Tar chemoreceptor. The plasmid is comprised of a promoter, a native RBS, Tar gene, a GFP gene and a terminator. In order to obtain the fusion, a proper linker sequence was introduced between the Tar and the GFP genes.
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==Usage and Biology==
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This device was used as a proof of concept in order to verify the migration of the chemoreceptor to the poles of the bacterial membrane.
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==Experiments and results==
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Expariment and resukt of this expraion system can be seen in the Tar-GFP part (<partinfo>K1992003</partinfo>) and in our resukt page [[http://2016.igem.org/Team:Technion_Israel/Tar_improvements Tar improvements and characterzation]]
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Revision as of 09:55, 21 October 2016


Tar GFP tagged, native RBS expression system (promoter+RBS+coding+terminator)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1355
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2397
    Illegal SapI.rc site found at 184

Introduction

A GFP gene was fused to the improved expression system of the Tar chemoreceptor. The plasmid is comprised of a promoter, a native RBS, Tar gene, a GFP gene and a terminator. In order to obtain the fusion, a proper linker sequence was introduced between the Tar and the GFP genes.

Usage and Biology

This device was used as a proof of concept in order to verify the migration of the chemoreceptor to the poles of the bacterial membrane.

Experiments and results

Expariment and resukt of this expraion system can be seen in the Tar-GFP part (BBa_K1992003) and in our resukt page http://2016.igem.org/Team:Technion_Israel/Tar_improvements Tar improvements and characterzation