Difference between revisions of "Part:BBa K2066061:Design"

 
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<partinfo>BBa_K2066061 short</partinfo>
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<partinfo>BBa_K2066061 SequenceAndFeatures</partinfo>
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===Design Notes===
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This part was designed to perform RiboJ characterization verifying and building on that performed in Lou et al. 2012 (“Ribozyme-based insulator parts buffer synthetic circuits from genetic context”). This part will be used in concert with WM16_015 (plLACO-1 Ribozyme Chracterization Part – cI GFP) to determine the influence of the RiboJ insulator sequence on gene expression.
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===Source===
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This part was created using Gibson Assembly methods. We used K2066035 as a template and an amplicon containing the relevant promoter sequence +/- RiboJ combination as the insert. RiboJ sequence sfGFP sequence from Lou et al., Supplement section V. sfGFP sequence derived and modified from Lou et al., Supplement section V. The UNS sequences are from Torella et al. 2013.
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===References===

Revision as of 22:23, 17 October 2016


Promoter characterization part: J23101 with RiboJ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 47
    Illegal NheI site found at 70
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 187


Design Notes

This part was designed to perform RiboJ characterization verifying and building on that performed in Lou et al. 2012 (“Ribozyme-based insulator parts buffer synthetic circuits from genetic context”). This part will be used in concert with WM16_015 (plLACO-1 Ribozyme Chracterization Part – cI GFP) to determine the influence of the RiboJ insulator sequence on gene expression.


Source

This part was created using Gibson Assembly methods. We used K2066035 as a template and an amplicon containing the relevant promoter sequence +/- RiboJ combination as the insert. RiboJ sequence sfGFP sequence from Lou et al., Supplement section V. sfGFP sequence derived and modified from Lou et al., Supplement section V. The UNS sequences are from Torella et al. 2013.


References