Difference between revisions of "Part:BBa K2130012"
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For this part, we have deleted the HNH domain of the SP-dCas9 and tested it's binding capability by assessing the strength in transcriptional activation via a VP64-p65-Rta. | For this part, we have deleted the HNH domain of the SP-dCas9 and tested it's binding capability by assessing the strength in transcriptional activation via a VP64-p65-Rta. | ||
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| + | <img class="content-img" src="https://static.igem.org/mediawiki/2016/8/8c/T--NTU-Singapore--NUC.png" alt="" style="width:600px; height:372px; box-shadow: none;"> | ||
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| + | <p style="font-size:18px; text-align: center;">Activation of ZsGreen reporter gene using NUC and REC truncations</p> | ||
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Revision as of 07:49, 19 October 2016
∆RuvCIII-2 ∆HNH Sp-dCas9
dCas9 is a catalytically inactive Cas9, which still retains it's ability to bind to DNA. For epigenetic regulation, the dCas9 is highly dependent upon the function of it's fusion partner.
For this part, we have deleted the HNH domain of the SP-dCas9 and tested it's binding capability by assessing the strength in transcriptional activation via a VP64-p65-Rta.
<img class="content-img" src="
" alt="" style="width:600px; height:372px; box-shadow: none;">
Activation of ZsGreen reporter gene using NUC and REC truncations
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 251
Illegal BglII site found at 1188
Illegal BamHI site found at 2006
Illegal XhoI site found at 2962 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2350
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2496
Illegal BsaI site found at 3158
Illegal BsaI.rc site found at 1411