Difference between revisions of "Part:BBa K1909005"
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<partinfo>BBa_K1909005 short</partinfo> | <partinfo>BBa_K1909005 short</partinfo> | ||
| − | + | BBa_K1909005 is a protein internal domain part for the design of EnvZ chimeric receptors. EnvZ is part of the well-studied and commonly used two-component system EnvZ/OmpR in Escherichia coli, offering a simple way of signal transduction. | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | It was shown that the periplasmic domain of osmolarity sensor protein EnvZ can be exchanged by ligand binding domains of other receptors without compromising its activity (ref. iGEM Tokyo 2012). For easy fusion protein design, we split EnvZ into two parts, removing the periplasmic ligand binding domain to be replaced by other ligand binding domains of interest. This part is designed to form the transmembrane signal transduction and the cytosolic kinase domains of EnvZ. It follows the EnvZ Head domain (BBa_K1909003) and a periplasmic ligand binding domain (e.g. BBa_K1909004). Upon activation of the ligand binding domain, the signal is thought to be transduced to the cytosolic histidine kinase domain, activating it. Once activated, EnvZ phosphorylates OmpR, which acts as a transcription factor activating expression by binding to the Omp Promoter (BBa_R0082). Activation may be monitored by using fluorescence reporters (BBa_K1909013 and BBa_K1909014). | ||
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<partinfo>BBa_K1909005 parameters</partinfo> | <partinfo>BBa_K1909005 parameters</partinfo> | ||
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Latest revision as of 21:57, 19 October 2016
EnvZ Signal Transduction and Kinase Domains
BBa_K1909005 is a protein internal domain part for the design of EnvZ chimeric receptors. EnvZ is part of the well-studied and commonly used two-component system EnvZ/OmpR in Escherichia coli, offering a simple way of signal transduction.
Usage and Biology
It was shown that the periplasmic domain of osmolarity sensor protein EnvZ can be exchanged by ligand binding domains of other receptors without compromising its activity (ref. iGEM Tokyo 2012). For easy fusion protein design, we split EnvZ into two parts, removing the periplasmic ligand binding domain to be replaced by other ligand binding domains of interest. This part is designed to form the transmembrane signal transduction and the cytosolic kinase domains of EnvZ. It follows the EnvZ Head domain (BBa_K1909003) and a periplasmic ligand binding domain (e.g. BBa_K1909004). Upon activation of the ligand binding domain, the signal is thought to be transduced to the cytosolic histidine kinase domain, activating it. Once activated, EnvZ phosphorylates OmpR, which acts as a transcription factor activating expression by binding to the Omp Promoter (BBa_R0082). Activation may be monitored by using fluorescence reporters (BBa_K1909013 and BBa_K1909014).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| chassis | E. coli JW 3367-3 |