Difference between revisions of "Part:BBa K1915003:Design"

 
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===Design Notes===
 
===Design Notes===
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This composite part was synthesized as a whole unit by IDT. Therefore there are no Biobrick scars. Instead a spacer "TCATAGAG" was included following R0011 and "TACTAGATG" following J61101 to improve translation.
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This composite part was synthesized as a whole unit by IDT. Therefore there are no Biobrick scars. Instead a spacer "TCATAGAG" was included following R0011 and "TACTAGATG" following J61101 to improve translation. There is an inducible lac promoter included for expression of the LTNF15 protein. The LTNF protein sequence has a BamHI restriction nuclease site in the very middle of it. This can be used for the purpose of diagnostic digest. Both a Myc and 6xHis tag are added on towards the end of the part. These tags are used to help purify and identify this protein after expression. The HRV 3C site has been added as a cleavage point to remove the two tags after purification of the protein.
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===Source===
 
===Source===

Latest revision as of 17:35, 29 October 2016


Inducible Lac promoter+LTNF15


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 177
    Illegal BamHI site found at 96
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This composite part was synthesized as a whole unit by IDT. Therefore there are no Biobrick scars. Instead a spacer "TCATAGAG" was included following R0011 and "TACTAGATG" following J61101 to improve translation. There is an inducible lac promoter included for expression of the LTNF15 protein. The LTNF protein sequence has a BamHI restriction nuclease site in the very middle of it. This can be used for the purpose of diagnostic digest. Both a Myc and 6xHis tag are added on towards the end of the part. These tags are used to help purify and identify this protein after expression. The HRV 3C site has been added as a cleavage point to remove the two tags after purification of the protein.

Source

This is a composite part that was assembled through gene synthesis from IDT.

References