Difference between revisions of "Part:BBa K1935022"
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+ | __NOTOC__ | ||
+ | <partinfo>BBa_K1935022 short</partinfo> | ||
+ | |||
+ | Ceanorhabditis elegans is an organism with a free-methylated cytosine genome. We looked for a worm’s promoter able to be regulated as mammalian CpG island. | ||
+ | <br> <br> | ||
+ | pSET-16 is a promoter of Caenorhabditis elegans classified as a HOT (Highly Occupied Target) regions. HOT regions have a rich G-C content that makes them similar to mammalian CpG islands. | ||
+ | Some HOT promoters have been mentioned by Chen and one of them, SET-16 (chromosome 3: 13643462-13643761), controls a non-essential gene. That’s why we chose it as a target to test epiCRISPR whithout killing the worm. | ||
+ | |||
+ | |||
+ | <!-- Add more about the biology of this part here | ||
+ | ===Usage and Biology=== | ||
+ | |||
+ | <!-- --> | ||
+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_K1935022 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | <!-- Uncomment this to enable Functional Parameter display | ||
+ | ===Functional Parameters=== | ||
+ | <partinfo>BBa_K1935022 parameters</partinfo> | ||
+ | <!-- --> |
Revision as of 16:24, 14 October 2016
SET-16 promoter in Caenorhabditis elegans
Ceanorhabditis elegans is an organism with a free-methylated cytosine genome. We looked for a worm’s promoter able to be regulated as mammalian CpG island.
pSET-16 is a promoter of Caenorhabditis elegans classified as a HOT (Highly Occupied Target) regions. HOT regions have a rich G-C content that makes them similar to mammalian CpG islands.
Some HOT promoters have been mentioned by Chen and one of them, SET-16 (chromosome 3: 13643462-13643761), controls a non-essential gene. That’s why we chose it as a target to test epiCRISPR whithout killing the worm.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]