Difference between revisions of "Part:BBa K1976049"
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+ | <h1>Colicin E2 immunity protein</h1> | ||
+ | Colicin E2 is a peptide, that is toxic to coliform bacteria. It is one of a group of proteins of this type, it being a nicking endonuclease. It has a corresponding activity inhibiting protein called immunity protein Im2, which is also in the registry under the name . | ||
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+ | <img style="width: 40%; height: 40%; margin-left: 15px; margin-right: 15px;" alt="" src="https://static.igem.org/mediawiki/parts/9/9b/Minicolicin.png"> | ||
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− | < | + | <b>Figure 1:</b>Structure of Colicin E2 DNase domain derived from a molecular dynamics simulation. <b>Note:</b> this is not actually this part but <a href="https://parts.igem.org/Part:BBa_K1976048">BBa_K1976048</a>, a 5 amino acids shorter version |
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+ | ===<h2>Usage</h2>=== | ||
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+ | The Colicin E2 immunity protein (<a href="https://parts.igem.org/Part:BBa_K1976027">BBa_K1976027</a> and <a href="https://parts.igem.org/Part:BBa_K1976028">BBa_K1976028</a>) can be used in combination with the Colicin E2 DNase. | ||
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+ | ===<h2>Characteristics</h2>=== | ||
− | < | + | <h2>Sequence and Features</h2> |
− | + | <partinfo>BBA_K1976049 SequenceAndFeatures</partinfo> | |
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+ | <b>References</b> | ||
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+ | [1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007<br> | ||
+ | </body> | ||
+ | </html> |
Revision as of 16:59, 23 October 2016
Colicin E2 immunity protein
Colicin E2 is a peptide, that is toxic to coliform bacteria. It is one of a group of proteins of this type, it being a nicking endonuclease. It has a corresponding activity inhibiting protein called immunity protein Im2, which is also in the registry under the name .![](https://static.igem.org/mediawiki/parts/9/9b/Minicolicin.png)
Figure 1:Structure of Colicin E2 DNase domain derived from a molecular dynamics simulation. Note: this is not actually this part but BBa_K1976048, a 5 amino acids shorter version
Usage
The Colicin E2 immunity protein (BBa_K1976027 and BBa_K1976028) can be used in combination with the Colicin E2 DNase.
Characteristics
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 46
- 1000COMPATIBLE WITH RFC[1000]
[1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007