Difference between revisions of "Part:BBa K1918108"
 
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<partinfo>BBa_K1918108 parameters</partinfo>
 
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<p>In the figures are the FACS test results of our parts., which are EBFP2 without and with intein respectively. The EBFP-A signals tell us that the split florescent proteins could fuse together and emit florescent light.</p>
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<p>Without Dox, constructs containing CMV show no florescent signal, which means that one single N- or C- terminal cannot light up. However, once the other half is induced, they bind and emit light.</p>
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Latest revision as of 22:05, 19 October 2016


CMV-Kozak-EBFP2N(1-154)

This part is consist of EBFP2N(1-154) and the CMV promoter. EBFP2N(1-154) is a peptied made up of the 1st(N-terminus) to 154th amino acids in fluorescent protein EBFP2. Driven by CMV promoter, the EBFP2N(1-154) could be expressed all time.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 756
    Illegal XbaI site found at 1236
    Illegal SpeI site found at 84
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 756
    Illegal SpeI site found at 84
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 756
    Illegal BamHI site found at 739
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 756
    Illegal XbaI site found at 1236
    Illegal SpeI site found at 84
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 756
    Illegal XbaI site found at 1236
    Illegal SpeI site found at 84
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 717


Template:Tsinghua-A

    In the figures are the FACS test results of our parts., which are EBFP2 without and with intein respectively. The EBFP-A signals tell us that the split florescent proteins could fuse together and emit florescent light.

    Without Dox, constructs containing CMV show no florescent signal, which means that one single N- or C- terminal cannot light up. However, once the other half is induced, they bind and emit light.