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Revision as of 12:36, 14 October 2016

mamP

The mamP gene from the magnetosome operon mamAB forms a protein homodimer. It is an iron-oxidase that contributes to the formation of magnetite by mediating on step in the process, the formation of iron(III)ferrihydrite.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 493
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

Usage: The mamP gene produces a protein that can be used to transfer electrons to iron molecules, specfically, in vitro it can form iron(III)ferrihydrite. It can be used in combination with other mam genes to form an electron transport complex that should form magnetite (Fe(II)Fe(III)2O4) crystals when the cell is exposed to iron in solution. It was used to assemble a construct of MamO, P, T and X in pSB1A3 for in vivo expression.

Biology: MamP has been described as a cytochrome protein due to the presence two c-type cytochrome domains, with haem binding domains. It forms a complex with other Mam proteins on the membrane of the native species, Magnetospirillum gryphiswaldense. Together they promote magnetite crystal maturation within an organelle called the magnetosome.

References

Siponen, Marina I. et al. "Structural Insight Into Magnetochrome-Mediated Magnetite Biomineralization". Nature 502.7473 (2013): 681-684.