Difference between revisions of "Part:BBa K1918101"
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<partinfo>BBa_K1918101 short</partinfo> | <partinfo>BBa_K1918101 short</partinfo> | ||
− | This part is consist of EBFP2N(1-154) and the TRE promoter. EBFP2N(1-154) is a peptied made up with the 1st(N-terminus) to 154th amino acids in fluorescent protein EBFP2. TRE is the Dox induced promoter. Driven by TRE promoter, the expression of | + | This part is consist of EBFP2N(1-154) and the TRE promoter. EBFP2N(1-154) is a peptied made up with the 1st(N-terminus) to 154th amino acids in fluorescent protein EBFP2. TRE is the Dox induced promoter. Driven by TRE promoter, the expression of EBFP2N(1-154) could be induced by Dox. |
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Revision as of 12:24, 14 October 2016
TRE-Kozak-EBFP2N(1-154)
This part is consist of EBFP2N(1-154) and the TRE promoter. EBFP2N(1-154) is a peptied made up with the 1st(N-terminus) to 154th amino acids in fluorescent protein EBFP2. TRE is the Dox induced promoter. Driven by TRE promoter, the expression of EBFP2N(1-154) could be induced by Dox.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 379
Illegal XbaI site found at 859 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 379
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 379
Illegal BamHI site found at 362
Illegal XhoI site found at 19 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 379
Illegal XbaI site found at 859 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 379
Illegal XbaI site found at 859 - 1000COMPATIBLE WITH RFC[1000]