Difference between revisions of "Part:BBa K1981101"
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===Usage and Biology===
 
===Usage and Biology===
 
===Characterization===
 
===Characterization===
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AI-2 Response Device consists of the AI-2 quorum sensor-inducible promoter BBa_K1981101(249), a GFP coding sequence BBa_E0040(747bp), a double terminator BBa_B0015(115). The total length of AI-2 Response Device A is 1111bp.
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[[Image:NKU China lsr Promoter 3 hour curve.png|800px|thumb|center|'''Figure 1:''' Colony PCR Verification for AI-2 Response Device A.]]
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AI-2 Response Device consists of the AI-2 quorum sensor-inducible promoter BBa_K1981101(249), a GFP coding sequence BBa_E0040(747bp), a double terminator BBa_B0015(115). The total length of AI-2 Response Device A is 1111bp.
 +
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[[Image:NKU China exogenously Added AI-2 On DeviceA NKU China.png|800px|thumb|center|'''Figure 1:''' GFP expression of AI-2 Response Device A when add exogenous AI-2.]]

Revision as of 23:29, 14 October 2016


lsr promoter of LuxS/AI-2 signaling pathway in E.coli

plsr long description

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

Characterization

AI-2 Response Device consists of the AI-2 quorum sensor-inducible promoter BBa_K1981101(249), a GFP coding sequence BBa_E0040(747bp), a double terminator BBa_B0015(115). The total length of AI-2 Response Device A is 1111bp.

Figure 1: Colony PCR Verification for AI-2 Response Device A.

AI-2 Response Device consists of the AI-2 quorum sensor-inducible promoter BBa_K1981101(249), a GFP coding sequence BBa_E0040(747bp), a double terminator BBa_B0015(115). The total length of AI-2 Response Device A is 1111bp.

Figure 1: GFP expression of AI-2 Response Device A when add exogenous AI-2.