|
|
| Line 15: |
Line 15: |
| | <I>HZAU-China</I> | | <I>HZAU-China</I> |
| | |width='60%' valign='top'| | | |width='60%' valign='top'| |
| − | | + | qqqq |
| − | Promoter cpcG2 is a 238bp green-light activated promoter from the genome of Synechocystis PCC6803. We tested the efficiency of the promoter by measuring the fluorescence of output sfGFP when bacteria are illuminated with green, red or no light. (For more detail, please view our wiki: http://2016.igem.org/Team:HZAU-China/Experiments)
| + | |
| − | | + | |
| − |
| + | |
| − | Fig.1 Mean sfGFP fluorescence of CcaS-CcaR system under green light, red light and darkness.
| + | |
| − | | + | |
| − | As is shown in the figure, in E.coli strain JT2, PcpcG2 produces 266.0 ± 19.3 and 509.0 ± 55.4 au of sfGFP in red and green light, corresponding to 1.91 ± 0.34-fold activation, demonstrating that PcpcG2 is functionally regulated by light, green-activated and red-repressed.
| + | |
| − | | + | |
| − | However, the figure also shows that that leaked expression is severe in dark surroundings. To better respond to red and green light, we optimized the promoter by refactoring it and created an innovative promoter, PcpcG2-172, with the truncation of a constitutive promoter within the cpcG2 promoter (BBa_K2012015. For more detail, please view our wiki: http://2016.igem.org/Team:HZAU-China/Experiments).
| + | |
| − | | + | |
| − | | + | |
| | |}; | | |}; |
| | <!-- End of the user review template --> | | <!-- End of the user review template --> |
| | <!-- DON'T DELETE --><partinfo>BBa_K592003 EndReviews</partinfo> | | <!-- DON'T DELETE --><partinfo>BBa_K592003 EndReviews</partinfo> |
Revision as of 12:04, 14 October 2016
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K592003
User Reviews
UNIQ68bd12ac79f89191-partinfo-00000000-QINU
UNIQ68bd12ac79f89191-partinfo-00000001-QINU
