Difference between revisions of "Part:BBa K1893011:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | This sequence was synthesised as a block with BioBrick Prefix on the 5' end and BioBrick Suffix on the 3' end. | |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | + | Anti-STAR is a sequence that Imperial iGEM 2016 have designed for the first time. It is identical to part of the STAR-target attenuator sequence (pAD1.S5) but much shorter in length. When transcribed, it binds to the STAR-antisense RNA (pAD1.A5) with much higher affinity than the STAR-target sequence. | |
| − | + | ||
===References=== | ===References=== | ||
| + | Imperial iGEM 2016 | ||
Revision as of 11:29, 23 October 2016
Constitutive anti-STAR (J23119+anti-STAR)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This sequence was synthesised as a block with BioBrick Prefix on the 5' end and BioBrick Suffix on the 3' end.
Source
Anti-STAR is a sequence that Imperial iGEM 2016 have designed for the first time. It is identical to part of the STAR-target attenuator sequence (pAD1.S5) but much shorter in length. When transcribed, it binds to the STAR-antisense RNA (pAD1.A5) with much higher affinity than the STAR-target sequence.
References
Imperial iGEM 2016