Difference between revisions of "Part:BBa K1893002:Experience"

Revision as of 20:25, 22 October 2016

We used this part to engineer a bacterial communication system which was bidirectional, so that two different strains could grow together. Each strain in our system must have a distinct HSL associated with it, so we required two quorum sensing systems which were orthogonal. We found that Las and Rhl had very little crosstalk, indicating that they were good candidates for our system.

This part worked as expected, and when we placed GFP downstream of the RhlR promoter, we saw fluorescence when we induced with C4 AHL.

Applications of BBa_K1893002

User Reviews

UNIQbfeaaeebce6d2b05-partinfo-00000000-QINU UNIQbfeaaeebce6d2b05-partinfo-00000001-QINU

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 __NOTOC__
-This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
+We used this part to engineer a bacterial communication system which was bidirectional, so that two different strains could grow together. Each strain in our system must have a distinct HSL associated with it, so we required two quorum sensing systems which were orthogonal. We found that Las and Rhl had very little crosstalk, indicating that they were good candidates for our system.
-how you used this part and how it worked out.
+This part worked as expected, and when we placed GFP downstream of the RhlR promoter, we saw fluorescence when we induced with C4 AHL.
 ===Applications of BBa_K1893002===