Difference between revisions of "Part:BBa K1919301"

Revision as of 10:12, 14 October 2016

livF

LivF encodes a subunit of leucine ABC tansporter. The LivF predicted protein sequence contains Walker A and Walker B motifs involved in the binding and hydrolysis of ATP, and the ABC signature motif (also called the linker peptide or C-loop). The conserved ‘signature motif’ can be identified in the nucleotide-binding domains of all ABC transporters, and may play a role in ATP hydrolysis. This device constructed in our team contains a promoter J23100, a RBS, livF coding region and a terminator. The device can be transferred into E.coli and overexpress livF directly. The expression of the livF RNA in the part has been studied via qPCR.The quantified result suggests that compared with negtive control of E.coli (not cantain the plasmid), livJ has been overexpressed in E.coli BL21 and E.coli DH5a.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1919301 short</partinfo>
-LivF encodes a subunit of leucine ABC tansporter. This device constructed in our team contains a promoter J23100, a RBS, livF coding region and a terminator. The device can be transferred into E.coli and overexpress livF directly. The expression of the livF RNA in the part has been studied via qPCR.The quantified result suggests that compared with negtive control of E.coli (not cantain the plasmid), livJ has been overexpressed in E.coli BL21 and E.coli DH5a.
+LivF encodes a subunit of leucine ABC tansporter. The LivF predicted protein sequence contains Walker A and Walker B motifs involved in the binding and hydrolysis of ATP, and the ABC signature motif (also called the linker peptide or C-loop). The conserved ‘signature motif’ can be identified in the nucleotide-binding domains of all ABC transporters, and may play a role in ATP hydrolysis. This device constructed in our team contains a promoter J23100, a RBS, livF coding region and a terminator. The device can be transferred into E.coli and overexpress livF directly. The expression of the livF RNA in the part has been studied via qPCR.The quantified result suggests that compared with negtive control of E.coli (not cantain the plasmid), livJ has been overexpressed in E.coli BL21 and E.coli DH5a.
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