Difference between revisions of "Part:BBa K1918108:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | AIM: EBFP2N(1-154) could be expressed in cell all time; | |
+ | ELEMENTS: EBFP2N(1-154), CMV | ||
===Source=== | ===Source=== |
Revision as of 12:41, 14 October 2016
CMV-Kozak-EBFP2N(1-154)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 756
Illegal XbaI site found at 1236
Illegal SpeI site found at 84 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 756
Illegal SpeI site found at 84 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 756
Illegal BamHI site found at 739 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 756
Illegal XbaI site found at 1236
Illegal SpeI site found at 84 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 756
Illegal XbaI site found at 1236
Illegal SpeI site found at 84 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 717
Design Notes
AIM: EBFP2N(1-154) could be expressed in cell all time; ELEMENTS: EBFP2N(1-154), CMV
Source
[pwx240(KpnI&XbaI)] [pwx235(KpnI&XbaI)]