Difference between revisions of "Part:BBa K1918106"
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<partinfo>BBa_K1918106 short</partinfo> | <partinfo>BBa_K1918106 short</partinfo> | ||
− | This part is consist of | + | This part is consist of EBFP2N(1-154):IntN and the CMV promoter. EBFP2N(1-154):IntN is a peptied made up of the 1st(N-terminus) to 154th amino acids in fluorescent protein EBFP2 and IntN. Driven by CMV promoter, the EBFP2N(1-154):IntN could be expressed all time. |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 12:34, 14 October 2016
CMV-Kozak-EBFP2N(1-154):IntN
This part is consist of EBFP2N(1-154):IntN and the CMV promoter. EBFP2N(1-154):IntN is a peptied made up of the 1st(N-terminus) to 154th amino acids in fluorescent protein EBFP2 and IntN. Driven by CMV promoter, the EBFP2N(1-154):IntN could be expressed all time.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 755
Illegal XbaI site found at 1499
Illegal SpeI site found at 83 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 755
Illegal SpeI site found at 83 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 755
Illegal BamHI site found at 738 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 755
Illegal XbaI site found at 1499
Illegal SpeI site found at 83 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 755
Illegal XbaI site found at 1499
Illegal SpeI site found at 83 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 716