Difference between revisions of "Part:BBa K2066040:Design"

 
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===Source===
 
===Source===
  
−
to be completed
+
The UNS2, UNS3, and UNS6 sequences are taken from Torella et al. 2013 (“Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly”). The spacer sB is taken from Pothoulakis et al. 2013 (“The Spinach RNA Aptamer as a Characterization Tool for Synthetic Biology”). The broccoli sequence is from https://www.addgene.org/66843/. The mCherry sequence is taken from WM16_029 with the start codon and extra stop codon added.
  
 
===References===
 
===References===

Revision as of 17:47, 16 October 2016


J23100 + RiboJ Promoter Characterization Part with 2x Broccoli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 47
    Illegal NheI site found at 70
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

to be completed


Source

The UNS2, UNS3, and UNS6 sequences are taken from Torella et al. 2013 (“Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly”). The spacer sB is taken from Pothoulakis et al. 2013 (“The Spinach RNA Aptamer as a Characterization Tool for Synthetic Biology”). The broccoli sequence is from https://www.addgene.org/66843/. The mCherry sequence is taken from WM16_029 with the start codon and extra stop codon added.

References