Difference between revisions of "Part:BBa K2086002:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | Design of the PyeaR-serA BioBrick was accomplished by combining our own serA BioBrick with the yeaR promoter and RBS used previously. Due to the inability to obtain just the yeaR promoter and RBS, a fragment that contained the yeaR promoter and RBS was synthesized by IDT with the BioBrick Prefix and suffix and then ligated to our own BioBrick. The terminator was from the part BBa_B0015 found in the distribution kit. All three parts were ligated together using 3A assembly. The terminator and serA were ligated first, and once that was completed the promoter and RBS were attached. | |
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===Source=== | ===Source=== | ||
Latest revision as of 16:40, 17 October 2016
PyeaR - SerA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 422
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Design of the PyeaR-serA BioBrick was accomplished by combining our own serA BioBrick with the yeaR promoter and RBS used previously. Due to the inability to obtain just the yeaR promoter and RBS, a fragment that contained the yeaR promoter and RBS was synthesized by IDT with the BioBrick Prefix and suffix and then ligated to our own BioBrick. The terminator was from the part BBa_B0015 found in the distribution kit. All three parts were ligated together using 3A assembly. The terminator and serA were ligated first, and once that was completed the promoter and RBS were attached.