Difference between revisions of "Part:BBa K2086002"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K2086002 short</partinfo> | <partinfo>BBa_K2086002 short</partinfo> | ||
| − | + | This part is a composite part of the promoter <partinfo>K216005</partinfo> with the serine coding sequence <partinfo>K2086002</partinfo> as well as a standard RBS <partinfo>BBa_B0030</partinfo> and terminator <partinfo>B0015</partinfo>. It is designed to act as a nitrate sensitive kill-switch by utilizing a nitrate sensitive promoter coordinated with an essential gene to complement an auxotrophic strain of E. coli only under high nitrate conditions. | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K2086002 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2086002 SequenceAndFeatures</partinfo> | ||
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| + | ===Biology and Our Application=== | ||
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Revision as of 19:00, 17 October 2016
PyeaR - SerA
This part is a composite part of the promoter BBa_K216005 with the serine coding sequence BBa_K2086002 as well as a standard RBS BBa_B0030 and terminator BBa_B0015. It is designed to act as a nitrate sensitive kill-switch by utilizing a nitrate sensitive promoter coordinated with an essential gene to complement an auxotrophic strain of E. coli only under high nitrate conditions.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 422
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]