Difference between revisions of "Part:BBa K2139002:Experience"

 
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===Applications of BBa_K2139002===
 
===Applications of BBa_K2139002===
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                        <img src="" style="width:50px; height:70px">
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                        </p>
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                        <p>
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                        <b>Figure 1.</b> (top) Western Blot of <i>C. crescentus</I> cellulase expressing strains, ran on SDS-PAGE and blotted with anti-RsaA. Left to right: (1) Thermofisher ladder, (2) Gluc1C cell lysate, (3) Endo5A cell lysate,
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                        (4) E1_399 cell lysate, (5) E1_422 cell lysate, (6) negative control  ΔGCSS (Δ<i>rsaA</i>) cell lysate, (7) P4A723 (wildtype) cell lysate, (8) Thermofisher ladder, (9)
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                        Gluc1C low pH extracted proteins, (10) Endo5A low pH extracted proteins, (11) E1_399 low pH extracted proteins, (12)
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                        E1_422 low pH extracted proteins, (13) ΔGCSS (Δ<i>rsaA</i>) low pH extracted proteins, (14) P4A723 (wildtype) low pH extracted proteins. (bottom) SDS-PAGE stained with coomassie brilliant blue, samples loaded in the same order as above for the Western Blot.
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                    <p style="text-align:center">
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                        <img src="" style="width:700px">
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                    </p>
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                    <p><b>Figure 2.</b> Assay for cellulase activity with DNPC substrate.</p>
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                        <img src="" style="width:600px">
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                    </p>
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                    <p><b>Figure 3.</b> Cellulase activity assay results for <i>C. crescentus</i> displaying cellulases compared to <i>C. crescentus</i> expressing wildtype RsaA (P4A723).</p>
  
 
===User Reviews===
 
===User Reviews===

Revision as of 02:00, 29 October 2016


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Please enter how you used this part and how it worked out.

Applications of BBa_K2139002

<img src="" style="width:50px; height:70px">

Figure 1. (top) Western Blot of C. crescentus cellulase expressing strains, ran on SDS-PAGE and blotted with anti-RsaA. Left to right: (1) Thermofisher ladder, (2) Gluc1C cell lysate, (3) Endo5A cell lysate, (4) E1_399 cell lysate, (5) E1_422 cell lysate, (6) negative control ΔGCSS (ΔrsaA) cell lysate, (7) P4A723 (wildtype) cell lysate, (8) Thermofisher ladder, (9) Gluc1C low pH extracted proteins, (10) Endo5A low pH extracted proteins, (11) E1_399 low pH extracted proteins, (12) E1_422 low pH extracted proteins, (13) ΔGCSS (ΔrsaA) low pH extracted proteins, (14) P4A723 (wildtype) low pH extracted proteins. (bottom) SDS-PAGE stained with coomassie brilliant blue, samples loaded in the same order as above for the Western Blot.

<img src="" style="width:700px">

Figure 2. Assay for cellulase activity with DNPC substrate.

<img src="" style="width:600px">

Figure 3. Cellulase activity assay results for C. crescentus displaying cellulases compared to C. crescentus expressing wildtype RsaA (P4A723).

User Reviews

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