Difference between revisions of "Part:BBa K1943017:Design"

 
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===Design Notes===
 
===Design Notes===
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This year we use mammalian cells as carriers. So in our plasmids, there are several kinds of anti-antibiotics genes which are used for screening. Usually, we will not use anti-antibiotics genes individually. We often use them together with some core protein such as GECO. However, it is monocistronic in the eukaryotic cells. There is a DNA sequence called 2A which connects two coding sequence and then both two call be expressed by the same promoter. We design primers and do PCR of this anti-antibiotics genes coding sequence together with 2A sequence and ligate it to pSB1C3 backbone.
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===Source===
 
===Source===

Revision as of 13:56, 16 October 2016


Puro+T2A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 481


Design Notes

This year we use mammalian cells as carriers. So in our plasmids, there are several kinds of anti-antibiotics genes which are used for screening. Usually, we will not use anti-antibiotics genes individually. We often use them together with some core protein such as GECO. However, it is monocistronic in the eukaryotic cells. There is a DNA sequence called 2A which connects two coding sequence and then both two call be expressed by the same promoter. We design primers and do PCR of this anti-antibiotics genes coding sequence together with 2A sequence and ligate it to pSB1C3 backbone.

Source

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References