Difference between revisions of "Part:BBa K1896013"
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<partinfo>BBa_K1896013 short</partinfo> | <partinfo>BBa_K1896013 short</partinfo> | ||
| − | + | This part was used to express a monomeric Streptavidin protein ([[Part:BBa_K1896000|mSA2]]) on the cell surface of ''E. coli''. The the [[Part:BBa_K1896003|INP<sub>NC</sub>]] domain, which consists of the N- and C-terminal domains of a ''Xanthomonas campestris'' ice nucleating protein, directs the fusion protein to the outer cell membrane. | |
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Revision as of 13:55, 19 October 2016
INP_NC-mSA2 generator
This part was used to express a monomeric Streptavidin protein (mSA2) on the cell surface of E. coli. The the INPNC domain, which consists of the N- and C-terminal domains of a Xanthomonas campestris ice nucleating protein, directs the fusion protein to the outer cell membrane.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 466
Illegal AgeI site found at 1082
Illegal AgeI site found at 1142 - 1000COMPATIBLE WITH RFC[1000]