Difference between revisions of "Part:BBa K2145000"
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<partinfo>BBa_K2145000 short</partinfo> | <partinfo>BBa_K2145000 short</partinfo> | ||
− | This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were | + | Reporter plasmid to demonstrate the effect of a random 500 bp spacer on reporter expression. This part contains two fluorescent protein coding sites (for RFP and GFP), both facing forward, and was assembled using Golden Gate Assembly. To make the plasmid, parts were amplified from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part. |
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Revision as of 22:41, 30 October 2016
GFP/RFP reporter (Forward/Forward) with random 500bp spacer
Reporter plasmid to demonstrate the effect of a random 500 bp spacer on reporter expression. This part contains two fluorescent protein coding sites (for RFP and GFP), both facing forward, and was assembled using Golden Gate Assembly. To make the plasmid, parts were amplified from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2226
Illegal AgeI site found at 2338 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 724