Difference between revisions of "Part:BBa K1941007:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | This version of the sgRNAs c6_TDH3 is the second closest gRNA possible to the transcription start site of the TDH3 promoter. It should therefore perform repression via CRISPRi when in complex with dCas9 as it will avoid the binding of the RNA polymerase. | |
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===Source=== | ===Source=== | ||
Latest revision as of 15:02, 19 October 2016
sgRNA c6_TDH3 (2)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 145
Illegal NgoMIV site found at 174 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This version of the sgRNAs c6_TDH3 is the second closest gRNA possible to the transcription start site of the TDH3 promoter. It should therefore perform repression via CRISPRi when in complex with dCas9 as it will avoid the binding of the RNA polymerase.
Source
some source!