Difference between revisions of "Part:BBa K2120321"

Revision as of 18:30, 22 October 2016

J23116+B0034+CI+B0015

J23116 is medium strong constitutive promoter，cI is an inhibitor which is built to repress the activity of pR promoter. We linked two elements by strong ribosome binding site(RBS) B0034 to build this device. The expression efficiency of cI depends on the concentration of plasmid within limits. So, we want to control the promoter strength to influence the expression efficiency of inhibitor.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 19:59, 13 October 2016 (view source) ZLRRR (Talk \| contribs) ← Older edit		Revision as of 18:30, 22 October 2016 (view source) Beaz (Talk \| contribs) Newer edit →
Line 3:		Line 3:
	<partinfo>BBa_K2120321 short</partinfo>		<partinfo>BBa_K2120321 short</partinfo>

−	~~J23119、J23116、J23109、J23106 are all~~ constitutive ~~promoter ,CI~~ is an inhibitor which is built to repress the ~~expression~~ of PR promoter. ~~The function is similar with the Part[BBa_K2120313]~~. The expression efficiency of CI depends on the concentration of plasmid within limits. So, we want to control the promoter strength to influence the expression efficiency of inhibitor.	+	J23116 is medium strong constitutive promoter，''cI'' is an inhibitor which is built to repress the activity of pR promoter. We linked two elements by strong ribosome binding site(RBS) B0034 to build this device. The expression efficiency of ''cI'' depends on the concentration of plasmid within limits. So, we want to control the promoter strength to influence the expression efficiency of inhibitor.