Difference between revisions of "Part:BBa K2120318"

Latest revision as of 18:23, 22 October 2016

J23109+B0032+tetR+B0015

J23109 is weak constitutive promoter，tetR is an inhibitor which is built to repress the activity of pTet promoter. We linked two elements by medium ribosome binding site(RBS) B0032 to build this device. The expression efficiency of tetR depends on the concentration of plasmid within limits. So, we want to control the promoter strength to influence the expression efficiency of inhibitor.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 19:58, 13 October 2016 (view source) ZLRRR (Talk \| contribs) ← Older edit		Latest revision as of 18:23, 22 October 2016 (view source) Beaz (Talk \| contribs)
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	<partinfo>BBa_K2120318 short</partinfo>		<partinfo>BBa_K2120318 short</partinfo>

−	~~J23119、J23116、J23109、J23106 are all~~ constitutive ~~promoter ,~~tetR is an inhibitor which is built to repress the ~~expression~~ of ~~Ptet~~ promoter. ~~The function is similar with the Part[ BBa_K2120314].~~. The expression efficiency of tetR depends on the concentration of plasmid within limits. So, we want to control the promoter strength to influence the expression efficiency of inhibitor.	+	J23109 is weak constitutive promoter，''tetR'' is an inhibitor which is built to repress the activity of pTet promoter. We linked two elements by medium ribosome binding site(RBS) B0032 to build this device. The expression efficiency of ''tetR'' depends on the concentration of plasmid within limits. So, we want to control the promoter strength to influence the expression efficiency of inhibitor.