Difference between revisions of "Part:BBa K2120307"

Revision as of 17:16, 22 October 2016

B0015+tetR+B0034+pBAD-AraC+B0015

We reserve K2120303BBa_K2120303 and ligate B0015 at the end of araC. This device is designed to construct K2120311.BBa_K2120311

Sequence and Features

Assembly Compatibility:

Revision as of 19:53, 13 October 2016 (view source) ZLRRR (Talk \| contribs) ← Older edit		Revision as of 17:16, 22 October 2016 (view source) Beaz (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K2120307 short</partinfo>		<partinfo>BBa_K2120307 short</partinfo>

−	~~pBAD is a promoter induced by arabinose~~ and ~~arac is~~ the ~~repressor. We use it to test the expression~~ of ~~reporter gene in our validation circuit~~ .~~And tetR~~ is ~~an inhibitor which is built~~ to ~~repress the expression of Ptet promoter~~. ~~We use it to control the “on” or ”off” state of toxin gene~~. ~~In order to avoid leaking out, We reserve 【BBa_K2120303 】~~.	+	We reserve K2120303[https://parts.igem.org/Part:BBa_K2120303 BBa_K2120303] and ligate B0015 at the end of ''araC''. This device is designed to construct K2120311.[https://parts.igem.org/Part:BBa_K2120311 BBa_K2120311]