Difference between revisions of "Part:BBa K1896007"

Latest revision as of 09:15, 19 October 2016

mGFPuv2 (C-part)

Variant of Part:BBa_K1896001 where the start codon was replaced by a GSTGS linker so it can be used in C-terminal protein fusions.

mGFPuv2 contains the following mutations:

F99S/M153T/V163A: GFPuv or cycle 3 GFP was optimised for excitation by UV light (360-400nm). [1]
S208L: Increases the fluorescence intensity of GFPuv, resulting in GFPuv2. [2]
A206K: Monomerizes most GFP variants by disrupting a hydrophobic patch. [3]

Sequence and Features

Assembly Compatibility:

von Stetten, D., Noirclerc-Savoye, M., Goedhart, J., Gadella, T. W., & Royant, A. (2012). Structure of a fluorescent protein from Aequorea victoria bearing the obligate-monomer mutation A206K. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 68(8), 878-882.
Ito, Y., Suzuki, M., & Husimi, Y. (1999). A novel mutant of green fluorescent protein with enhanced sensitivity for microanalysis at 488 nm excitation. Biochemical and biophysical research communications, 264(2), 556-560.
Crameri, A., Whitehorn, E. A., Tate, E., Stemmer, W. P., Crameri, A., Kitts, P. A., & Kitts, P. A. (1996). Improved green fluorescent protein by molecular evolution using. Nat. Biotechnol, 14(3), 315-319.

@@ Line 7: / Line 7: @@
 mGFPuv2 contains the following mutations:
 * '''F99S/M153T/V163A''': GFPuv or ''cycle 3'' GFP was optimised for excitation by UV light (360-400nm). [1]
-* '''S208L''': Almost doubles the fluorescent intensity of GFPuv, resulting in GFPuv2. [2]
+* '''S208L''': Increases the fluorescence intensity of GFPuv, resulting in GFPuv2. [2]
 * '''A206K''': Monomerizes most GFP variants by disrupting a hydrophobic patch. [3]