Difference between revisions of "Part:BBa K1937005:Design"
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===Design Notes=== | ===Design Notes=== | ||
The BBa_K1937005 part was designed to demonstrate the induction of pNagP by NAG. It belongs to the antifungal module in the Paleolitis project of iGEM Toulouse 2016 (http://2016.igem.org/Team:Toulouse_France). | The BBa_K1937005 part was designed to demonstrate the induction of pNagP by NAG. It belongs to the antifungal module in the Paleolitis project of iGEM Toulouse 2016 (http://2016.igem.org/Team:Toulouse_France). | ||
− | The part includes the pNagP promoter and its RBS (position 840,455 to 840,655 of the Bacillus genome). The promoter controls the expression of the RFP fluorescent reporter gene. A NheI restriction site has been placed before the RFP ORF to facilitate promoter swapping. | + | The part includes the pNagP promoter and its RBS (position 840,455 to 840,655 of the <i>Bacillus</i> genome). The promoter controls the expression of the RFP fluorescent reporter gene. A NheI restriction site has been placed before the RFP ORF to facilitate promoter swapping. |
[[File:BBa_K1937005-map.png]] | [[File:BBa_K1937005-map.png]] | ||
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===Source=== | ===Source=== | ||
− | In | + | In <i>Bacillus subtilis</i>, this promoter has been reported to induce expression in presence of N-acetylglucosamine 6-phosphate. |
===References=== | ===References=== |
Revision as of 10:06, 17 October 2016
pNagP-RFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 201
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 761
Illegal AgeI site found at 873 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The BBa_K1937005 part was designed to demonstrate the induction of pNagP by NAG. It belongs to the antifungal module in the Paleolitis project of iGEM Toulouse 2016 (http://2016.igem.org/Team:Toulouse_France). The part includes the pNagP promoter and its RBS (position 840,455 to 840,655 of the Bacillus genome). The promoter controls the expression of the RFP fluorescent reporter gene. A NheI restriction site has been placed before the RFP ORF to facilitate promoter swapping.
Source
In Bacillus subtilis, this promoter has been reported to induce expression in presence of N-acetylglucosamine 6-phosphate.