Difference between revisions of "Part:BBa K1937004:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | The part includes the pNagA promoter and its RBS (position 3,595,156 to 3,595,356 of the Bacillus genome). The promoter controls the expression of the RFP fluorescent reporter gene. A NheI restriction site has been placed before the RFP ORF to facilitate promoter swapping.This part is a subcloning of BBa_K1937003 pNagA part in pSBBS0K_mini plasmid (BBa_K1937001). | + | The part includes the pNagA promoter and its RBS (position 3,595,156 to 3,595,356 of the <i>Bacillus</i> genome). The promoter controls the expression of the RFP fluorescent reporter gene. A NheI restriction site has been placed before the RFP ORF to facilitate promoter swapping.This part is a subcloning of BBa_K1937003 pNagA part in pSBBS0K_mini plasmid (BBa_K1937001). |
Here is a map of the gene: | Here is a map of the gene: | ||
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===Source=== | ===Source=== | ||
− | The part include the pNagA promoter and its RBS (position 3,595,156 to 3,595,356 of the Bacillus genome). The promoter controls the expression of the RFP fluorescent reporter gene. A NheI restriction site has been placed before the RFP ORF to facilitate promoter swapping.This part is a subcloning of BBa_K1937003 pNagA part in pSBBS0K_mini plasmid (BBa_K1937001). | + | The part include the pNagA promoter and its RBS (position 3,595,156 to 3,595,356 of the <i>Bacillus</i> genome). The promoter controls the expression of the RFP fluorescent reporter gene. A NheI restriction site has been placed before the RFP ORF to facilitate promoter swapping.This part is a subcloning of BBa_K1937003 pNagA part in pSBBS0K_mini plasmid (BBa_K1937001). |
− | In | + | In <i>Bacillus subtilis</i>, this promoter has been reported to induce expression in presence of N-acetylglucosamine 6-phosphate. |
===References=== | ===References=== |
Revision as of 10:05, 17 October 2016
pNagA-RFP in pSBBs0K-mini plasmid
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1257
Illegal suffix found in sequence at 1285 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1257
Illegal NheI site found at 201
Illegal NheI site found at 1279
Illegal SpeI site found at 1286
Illegal PstI site found at 1300
Illegal NotI site found at 1263
Illegal NotI site found at 1293 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1257
Illegal BglII site found at 999
Illegal BglII site found at 5814
Illegal BamHI site found at 1330 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1257
Illegal suffix found in sequence at 1286 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1257
Illegal XbaI site found at 1272
Illegal SpeI site found at 1286
Illegal PstI site found at 1300
Illegal NgoMIV site found at 2877
Illegal AgeI site found at 761
Illegal AgeI site found at 873
Illegal AgeI site found at 5425
Illegal AgeI site found at 6387 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1055
Illegal BsaI.rc site found at 2701
Illegal BsaI.rc site found at 4140
Illegal BsaI.rc site found at 6656
Illegal SapI site found at 1618
Illegal SapI.rc site found at 5638
Design Notes
The part includes the pNagA promoter and its RBS (position 3,595,156 to 3,595,356 of the Bacillus genome). The promoter controls the expression of the RFP fluorescent reporter gene. A NheI restriction site has been placed before the RFP ORF to facilitate promoter swapping.This part is a subcloning of BBa_K1937003 pNagA part in pSBBS0K_mini plasmid (BBa_K1937001). Here is a map of the gene:
Source
The part include the pNagA promoter and its RBS (position 3,595,156 to 3,595,356 of the Bacillus genome). The promoter controls the expression of the RFP fluorescent reporter gene. A NheI restriction site has been placed before the RFP ORF to facilitate promoter swapping.This part is a subcloning of BBa_K1937003 pNagA part in pSBBS0K_mini plasmid (BBa_K1937001).
In Bacillus subtilis, this promoter has been reported to induce expression in presence of N-acetylglucosamine 6-phosphate.