Difference between revisions of "Part:BBa K2150112"

 
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<h2>toxin N-acetyltransferase GCN5 induced by aTc</h2>
  
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__NOTOC__
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===Usage and Biology===
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<partinfo>BBa_K2150112 short</partinfo>
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a
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This part is used to build a kill-switch.
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===Characterization===
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<h4>Growth Curve</h4>
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We measured the growth curve of bacteria expressing toxin N-acetyltransferase GCN5.
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[[file:T--UCAS--136t-atc.png|500px]]
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The growth curve of wild type E. coli harboring empty vector is shown below.
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[[file:T--UCAS--RGP_Ptet.png|500px]]
  
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
  
 
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Revision as of 02:03, 19 October 2016

toxin N-acetyltransferase GCN5 induced by aTc

Usage and Biology

This part is used to build a kill-switch.

Characterization

Growth Curve

We measured the growth curve of bacteria expressing toxin N-acetyltransferase GCN5.

T--UCAS--136t-atc.png

The growth curve of wild type E. coli harboring empty vector is shown below.

T--UCAS--RGP Ptet.png


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 240
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 486
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 240
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 240
    Illegal NgoMIV site found at 417
  • 1000
    COMPATIBLE WITH RFC[1000]