Difference between revisions of "Part:BBa K2120309"

Revision as of 18:49, 13 October 2016

B0015+CI+B0034+pBAD-AraC+pR+B0032+RFP+B0015 pBAD is a promoter induced by arabinose and arac is the repressor. We use it to test the expression of reporter gene in our validation circuit .And LacIq is an inhibitor which is built to repress the expression of PLac promoter. We use it to control the “on” or ”off” state of toxin gene.YFP is the reporter gene. In order to test these do not interfere with each other , We reserve 【BBa_K2120304 】.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1001
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1166
Illegal AgeI site found at 2788
Illegal AgeI site found at 2900
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1183

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K2120309 short</partinfo>
+pBAD is a promoter induced by arabinose and arac is the repressor. We use it to test the expression of reporter gene in our validation circuit .And LacIq is an inhibitor which is built to repress the expression of PLac promoter. We use it to control the “on” or ”off” state of toxin gene.YFP is the reporter gene. In order to test these do not interfere with each other , We reserve 【BBa_K2120304 】.