Difference between revisions of "Part:BBa K1997016:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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This part is designed to be a negative control which contains the split ZFN protein.
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We added BsaI sites at the flank of "FRB-RBS-FKBP" sequence so that this sequence can be easily replaced
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+
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+
  
 
===Source===
 
===Source===

Revision as of 14:06, 13 October 2016


P+R->sGFP-N->Zif-N->RBS->Zif-C->sGFP-C->TER


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 832
    Illegal AgeI site found at 916
    Illegal AgeI site found at 1036
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1039
    Illegal BsaI.rc site found at 736
    Illegal BsaI.rc site found at 1253


Design Notes

We added BsaI sites at the flank of "FRB-RBS-FKBP" sequence so that this sequence can be easily replaced

Source

We obtained it through the contribution of our basic subparts.

References