Difference between revisions of "Part:BBa K1997013"
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<partinfo>BBa_K1997013 short</partinfo> | <partinfo>BBa_K1997013 short</partinfo> | ||
− | This part is | + | This part is an integrated tool for protein-protein interaction research using split-HRP system as reporter. the "Zif-N->RBS->Zif-C" subpart can be easily replaced using Golden Gate technique with BsaI |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 00:26, 14 October 2016
P+R->sHRP-N->Zif-N->RBS->Zif-C->sHRP-C->TER
This part is an integrated tool for protein-protein interaction research using split-HRP system as reporter. the "Zif-N->RBS->Zif-C" subpart can be easily replaced using Golden Gate technique with BsaI
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 382
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 624
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1003
Illegal AgeI site found at 1087
Illegal AgeI site found at 1207 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1210
Illegal BsaI.rc site found at 907
Illegal SapI.rc site found at 1324