Difference between revisions of "Part:BBa K1997023"

 
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<partinfo>BBa_K1997023 short</partinfo>
 
<partinfo>BBa_K1997023 short</partinfo>
  
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This is a device which contains the sequence of split luciferase,FRB and FKBP. Through this device, we can test and verify the function of luciferase reporter system.
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This part is an integrated tool for protein-protein interaction research using split-LUC system as reporter. the "FRB-RBS-FKBP" subpart can be easily replaced using Golden Gate technique with BsaI
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 01:54, 14 October 2016


P+R->sLuc-N->FRB->RBS->FKBP->sLuc-C->TER

This part is an integrated tool for protein-protein interaction research using split-LUC system as reporter. the "FRB-RBS-FKBP" subpart can be easily replaced using Golden Gate technique with BsaI Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2158
    Illegal BsaI.rc site found at 1513