Difference between revisions of "Part:BBa K1997011:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | We added BsaI sites at the flank of "FRB-RBS-FKBP" sequence so that this sequence can be easily replaced | |
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===Source=== | ===Source=== | ||
Revision as of 04:17, 17 October 2016
P+R->sHRP-N->FRB->RBS->FKBP->sHRP-C->Ter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 383
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 625
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1553
Illegal BsaI.rc site found at 908
Illegal SapI.rc site found at 1667
Design Notes
We added BsaI sites at the flank of "FRB-RBS-FKBP" sequence so that this sequence can be easily replaced
Source
This part is assembled from K1789012, K1997000 and a dCas9 fragment