Difference between revisions of "Part:BBa K1830002:Experience"

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BBa K1830002 is stated as the signal peptide of Usp45, however,after thorough literature search we found that SPusp45 is only 27 amino acids long, but not the current version of BBa_K1830002. We confirmed that the 27 amino acids long SPusp45 signal peptide can be used for secreting proteins out of the L.lactis cell. It should be fused to the N-terminal of the protein of interest.
 
BBa K1830002 is stated as the signal peptide of Usp45, however,after thorough literature search we found that SPusp45 is only 27 amino acids long, but not the current version of BBa_K1830002. We confirmed that the 27 amino acids long SPusp45 signal peptide can be used for secreting proteins out of the L.lactis cell. It should be fused to the N-terminal of the protein of interest.
  
 +
===Group===
 +
TMMU-China-2016
 +
===Author===
 +
Yifei Lu
 +
===Summary===
 +
We characterized that the 27 amino acids long SPusp45 signal peptide but not BBa K1654007 is the right choice that can be used to secret proteins out of L. lactis.
 
===User Reviews===
 
===User Reviews===
 
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Revision as of 08:12, 13 October 2016


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1830002

BBa K1830002 is stated as the signal peptide of Usp45, however,after thorough literature search we found that SPusp45 is only 27 amino acids long, but not the current version of BBa_K1830002. We confirmed that the 27 amino acids long SPusp45 signal peptide can be used for secreting proteins out of the L.lactis cell. It should be fused to the N-terminal of the protein of interest.

Group

TMMU-China-2016

Author

Yifei Lu

Summary

We characterized that the 27 amino acids long SPusp45 signal peptide but not BBa K1654007 is the right choice that can be used to secret proteins out of L. lactis.

User Reviews

UNIQ0e95fe995686b601-partinfo-00000000-QINU UNIQ0e95fe995686b601-partinfo-00000001-QINU