Difference between revisions of "Part:BBa K1887003:Design"

(→‎References)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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Incorporation of the nsr gene into the nisin controlled expression system will help NZ9000 to tolerate high levels of nisin concentration.
+
The cA anchoring domain is used in surface display system for L. lactis.
  
 
===Source===
 
===Source===

Revision as of 07:35, 14 October 2016


cA anchoring domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The cA anchoring domain is used in surface display system for L. lactis.

Source

By DNA synthesis.

References

[1] Hacker, C., Christ, N.A., Duchardt-Ferner, E., Korn, S., Gobl, C., Berninger, L., et al. (2015). The Solution Structure of the Lantibiotic Immunity Protein NisI and Its Interactions with Nisin. J Biol Chem 290, 28869-28886.

[2] Khosa, S., Lagedroste, M., and Smits, S.H. (2016). Protein Defense Systems against the Lantibiotic Nisin: Function of the Immunity Protein NisI and the Resistance Protein NSR. Front Microbiol 7, 504.

[3] Sun, Z., Zhong, J., Liang, X., Liu, J., Chen, X., and Huan, L. (2009). Novel mechanism for nisin resistance via proteolytic degradation of nisin by the nisin resistance protein NSR. Antimicrob Agents Chemother 53, 1964-1973.