Difference between revisions of "Part:BBa K1916102:Design"

 
 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
Protein from cyanobacteria genome. pBAD promoter sequence of K206000, ribosome binding site sequence of B0034.
+
Protein from cyanobacteria genome (sequence provided with assistance of Nathan Rockwell and Clark Lagarias of the Lagarias Lab, UC Davis). pBAD promoter sequence of K206000, ribosome binding site sequence of B0034.
  
 
===References===
 
===References===

Latest revision as of 07:07, 25 October 2016


Ara-Inducible Cyan7427_1390g3 Expression System


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 731
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1251
    Illegal AgeI site found at 1341
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns.


Source

Protein from cyanobacteria genome (sequence provided with assistance of Nathan Rockwell and Clark Lagarias of the Lagarias Lab, UC Davis). pBAD promoter sequence of K206000, ribosome binding site sequence of B0034.

References