Difference between revisions of "Part:BBa K2150021"
| Line 5: | Line 5: | ||
This part is a complete regulatory device sensitive to tetracycline. | This part is a complete regulatory device sensitive to tetracycline. | ||
The first promoter(BBa_J23119) is constitutively on and can express tetR, a protein that can repress the downstream promoter pTet in the absence of tetracycline(Tc). As a result, no fluorescence can be detected. In the presence of Tc, however, tetR can be repressed by Tc and repression over pTet can be removed, resulting in the expression of GFP and T7RNAP. Under such circumstances, positive feedback can be achieved and obvious fluorescence can be observed. | The first promoter(BBa_J23119) is constitutively on and can express tetR, a protein that can repress the downstream promoter pTet in the absence of tetracycline(Tc). As a result, no fluorescence can be detected. In the presence of Tc, however, tetR can be repressed by Tc and repression over pTet can be removed, resulting in the expression of GFP and T7RNAP. Under such circumstances, positive feedback can be achieved and obvious fluorescence can be observed. | ||
| + | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 04:17, 13 October 2016
pCon+RBS+tetR+DT+pT7+pTet+RBS+GFP+RBS+T7RNAP+DT
This part is a complete regulatory device sensitive to tetracycline. The first promoter(BBa_J23119) is constitutively on and can express tetR, a protein that can repress the downstream promoter pTet in the absence of tetracycline(Tc). As a result, no fluorescence can be detected. In the presence of Tc, however, tetR can be repressed by Tc and repression over pTet can be removed, resulting in the expression of GFP and T7RNAP. Under such circumstances, positive feedback can be achieved and obvious fluorescence can be observed.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1643