Difference between revisions of "Part:BBa K1900001"
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<partinfo>BBa_K1900001 short</partinfo> | <partinfo>BBa_K1900001 short</partinfo> | ||
| − | + | Combination of part BBa_K1406000 (BBa_K206000 + BBa_B0034) the E. coli tolC signaling sequence and the Serratia marcescens tolC gene. Transcription is controlled by the arabinose-induced pBAD promoter (BBa_K206000). A strong E. coli RBS (BBa_B0034) controls translation. The signaling sequence is a cleavable N-terminal signaling sequence from E. coli's tolC gene. This is required for trimerization of the TolC monomers as well as insertion into the outer membrane; it forms a chimeric polypeptide with the tolC gene from Serratia marcescens. TolC trimers form a outer-membrane alpha/beta barrel pore can interface with a variety of molecule pumps to form a transperiplasmic pump.<!-- Add more about the biology of this part here | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 16:54, 20 October 2016
pBAD+strong RBS+E. coli tolC signal sequence+S. marcescens tolC
Combination of part BBa_K1406000 (BBa_K206000 + BBa_B0034) the E. coli tolC signaling sequence and the Serratia marcescens tolC gene. Transcription is controlled by the arabinose-induced pBAD promoter (BBa_K206000). A strong E. coli RBS (BBa_B0034) controls translation. The signaling sequence is a cleavable N-terminal signaling sequence from E. coli's tolC gene. This is required for trimerization of the TolC monomers as well as insertion into the outer membrane; it forms a chimeric polypeptide with the tolC gene from Serratia marcescens. TolC trimers form a outer-membrane alpha/beta barrel pore can interface with a variety of molecule pumps to form a transperiplasmic pump. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1642
Illegal NheI site found at 1663 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1582
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 337
Illegal NgoMIV site found at 1126
Illegal NgoMIV site found at 1413
Illegal AgeI site found at 1311 - 1000COMPATIBLE WITH RFC[1000]