Difference between revisions of "Part:BBa K1980003:Design"
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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This protein has a C terminal hexahistidine tag for purification and has been codon optimised for E. coli.
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This protein has a C terminal hexahistidine tag for purification and has been codon optimised for E. coli. The sfGFP has been seperated from the MymT by a short linker (GlySerGlySerGlySer) to allow both domains to fold.
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===Source===
 
===Source===

Revision as of 20:46, 23 October 2016


MymT sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 595
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This protein has a C terminal hexahistidine tag for purification and has been codon optimised for E. coli. The sfGFP has been seperated from the MymT by a short linker (GlySerGlySerGlySer) to allow both domains to fold.

Source

MymT is originally from Mycobacterium tuberculosis. We ordered it as codon optimised DNA from IDT.

References